MUTE-SIM: multiphoton up-conversion time-encoded structured illumination microscopy
نویسندگان
چکیده
منابع مشابه
Superresolution Structured Illumination Microscopy (SR-SIM)
How Superresolution with ELYRA S.1 works The resolving power of a light microscope is limited to approximately 200nm in the lateral (XY) and 500nm in the axial (Z) direction. This so-called diffraction limit has been fi rst described by Ernst Abbe in 1873 (reference 1) and still holds true today. In simple terms, it posits that objects cannot be resolved with a conventional light microscope if ...
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A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perime...
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In the last couple of decades, the spatial resolution in optical microscopy has increased to unprecedented levels by exploiting the fluorescence properties of the probe. At about the same time, Raman imaging techniques have emerged as a way to image inherent chemical information in a sample without using fluorescent probes. However, in many applications, the achievable resolution is limited to ...
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At the start of this millennium, the principles of structured illumination microscopy (SIM) had been established and the concept of resolution doubling demonstrated experimentally in two dimensions. Breathtaking advances have since taken place, making SIM one of the most powerful and versatile superresolution methods available today, routinely used in the study of biochemical processes in labor...
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ژورنال
عنوان ژورنال: OSA Continuum
سال: 2020
ISSN: 2578-7519
DOI: 10.1364/osac.387129